Acute toxicity and anticancer potential of knobweed (Hyptis capitata) ethanolic leaf extract and fraction

Ethanolic leaf extract of Hyptis capitata Jacq. showed a toxic effect on Artemia salina larvae1. The was simplified through fractionation using vacuum liquid chromatography to trace compounds with biological activity. F1, F2, F3 and F4 fractions were used in the acute toxicity test A. larvae. F1 highest value 196.77±2.7 µg/ml. As an initial screening for its anticancer potential, ethanolic fraction subjected cytotoxicity T47D cells obtain IC50 45.32±2.0 >1000 µg/mL. LC50 level decreased compared extract. Based GC-MS analysis, contained neophytadiene, hexadecanoic acid, methyl ester, ethyl ester heptadecanoic 16-methyl-, ester. Meanwhile, ?-sitosterol, docosanoic squalene are only found work synergistically. This implies has potential be developed as candidate.